RapidChek® CONFIRM™ Non-O157 STEC

The RapidChek® CONFIRM™ STEC IMS Kit is designed for the confirmation of the “Big Six” non-O157 and O157 STECs on selective agar in beef samples. The use of this kit increases the accuracy and repeatability and reduces the labor and time required for confirmation.

How the Test Works

The RapidChek® CONFIRM™ STEC IMS confirmation kit uses specific, high-affinity antibodies each attached to separate magnetic particles. Each coated magnetic particle solution is added to a potential positive enrichment and incubated. If any of the STECs are present, it will bind to the magnetic particles via the antibody. A magnet is then used to concentrate the bound magnetic particles and the remaining enrichment is discarded. When the sample is plated to selective agar for confirmation, E. coli O26, O45, O103, O111, O121, O145 and O157 are the predominant colonies that form increasing the likelihood of picking that organism from a plate. By reducing the presence of other bacterial species, the accuracy and repeatability of the cultural confirmation is increased and the labor and time decreased.

Validated Applications

The RapidChek® CONFIRM™ STEC IMS Kit is approved by the USDA FSIS in MLG 5B.04 for use in the confirmation of E. coli O26, O45, O103, O111, O121, O145 or O157 from potential positive beef samples.

Customer benefits

• Isolate E. coli O26, O45, O103, O111, O121, O145 and O157 present within an enriched sample
• Increases the chances of identifying a “Big Six” non-O157 and O157 STEC from selective agar plates
• Easy to use technology requiring no additional instrumentation
• Validated: Listed in the USDA FSIS MLG 5B.04 for the confirmation of non-O157 STECs